Identifying PR Genes in Apple and Their Response to Pathogens
Author Information
Author(s): Bonasera Jean M, Kim Jihyun F, Beer Steven V
Primary Institution: Cornell University
Hypothesis
The SAR pathway occurs in apple because genes related to the pathway are highly conserved across the plant kingdom.
Conclusion
Four PR genes from apple were partially characterized, and PR-1a, PR-1b, and PR-1c are not involved in defense response or SAR in young apple shoots.
Supporting Evidence
- PR-2, PR-5, and PR-8 were induced in response to inoculation with E. amylovora.
- PR-1a was not induced during the first 96 hours following inoculation with E. amylovora.
- None of the four apple PR genes were induced in response to treatment with elicitors.
Takeaway
Scientists studied apple trees to find genes that help them fight off diseases. They found some genes that work differently than expected when the trees are attacked by bacteria.
Methodology
The study involved identifying PR genes in apple using PCR and analyzing their expression following inoculation with E. amylovora.
Limitations
The study did not identify markers for SAR in apple, and the results may differ in more mature trees compared to seedlings.
Digital Object Identifier (DOI)
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