The relationship between transcription initiation RNAs and CCCTC-binding factor (CTCF) localization
2011
How tiny RNAs affect gene regulation and CTCF binding
publication
Evidence: moderate
Author Information
Author(s): Taft Ryan J, Hawkins Peter G, Mattick John S, Morris Kevin V
Hypothesis
Do transcription initiation RNAs (tiRNAs) influence the localization of CCCTC-binding factor (CTCF)?
Conclusion
The study suggests that tiRNAs modulate local epigenetic structure, which in turn regulates CTCF localization.
Supporting Evidence
- tiRNAs are enriched at CTCF binding sites in both human and mouse.
- Inhibition of tiRNAs near CTCF sites increases CTCF localization and p21 expression.
- Overexpression of tiRNA mimics decreases CTCF localization and p21 expression.
Takeaway
Tiny RNAs can help control how genes are turned on or off by affecting a protein that organizes DNA in the cell.
Methodology
The study involved examining the relationship between tiRNAs and CTCF binding through experiments in human and mouse cells.
Digital Object Identifier (DOI)
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