Increasing Retinoic Acid in Neuroblastoma Cells
Author Information
Author(s): Armstrong J L, Ruiz M, Boddy A V, Redfern C P F, Pearson A D J, Veal G J
Primary Institution: University of Newcastle Upon Tyne
Hypothesis
Can retinoids like acitretin and CYP26 inhibitors increase the intracellular availability of all-trans retinoic acid (ATRA) in neuroblastoma cells?
Conclusion
The study found that acitretin and R116010 can significantly increase intracellular levels of ATRA in neuroblastoma cells.
Supporting Evidence
- Incubation with acitretin significantly increased intracellular ATRA levels in SH-SY5Y cells.
- R116010 also significantly increased ATRA concentrations when combined with either ATRA or 13cisRA.
- CRABP expression was induced in response to retinoid treatment, correlating with increased ATRA levels.
Takeaway
This study shows that certain drugs can help more of a cancer-fighting vitamin get into cancer cells, which might help treat kids with a type of cancer called neuroblastoma.
Methodology
Neuroblastoma cell lines were treated with acitretin or R116010, and intracellular retinoid levels were measured using HPLC.
Potential Biases
Potential bias in the selection of cell lines and treatment concentrations.
Limitations
The study's findings may not be generalizable to all neuroblastoma cell lines due to varying responses to treatment.
Participant Demographics
The study used various neuroblastoma cell lines, including SH-SY5Y, SH-EP, IMR-32, and NGP.
Statistical Information
P-Value
p=0.03
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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