Role of Prion Protein in Erythropoiesis
Author Information
Author(s): Panigaj Martin, Glier Hana, Wildova Marcela, Holada Karel
Primary Institution: Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
Hypothesis
What is the role of the cellular prion protein (PrPC) in erythropoiesis?
Conclusion
The regulation of PrPC expression in differentiating MEL cells mimics the pattern detected on mouse erythroid precursors in vivo.
Supporting Evidence
- The expression of PrPC was highest on early erythroblasts and decreased with maturation.
- Silencing of PrPC did not affect MEL cell differentiation.
- PrPC levels were stably repressed during the differentiation of MEL cells.
Takeaway
This study looked at how a protein called PrPC behaves in blood cell development. It found that PrPC levels go up when cells are young and then go down as they mature.
Methodology
The study used quantitative multicolor flow cytometry, western blot analysis, and quantitative RT-PCR to analyze PrPC expression.
Statistical Information
Statistical Significance
p>0.005
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website