PNRC Stimulates RNA Polymerase III Transcription
Author Information
Author(s): Zhou Dujin, Zhong Shuping, Ye Jing-Jing, Quach Keith M, Johnson Deborah L, Chen Shiuan
Primary Institution: Beckman Research Institute of City of Hope and University of Southern California
Hypothesis
Does PNRC interact with RPC39 to stimulate RNA polymerase III-dependent transcription?
Conclusion
The study demonstrates that human PNRC stimulates RNA pol III transcription through its interaction with the subunit RPC39 of RNA pol III.
Supporting Evidence
- PNRC was identified as an interacting partner of RPC39 through yeast two-hybrid screening.
- Co-immunoprecipitation confirmed the interaction between PNRC and RPC39 in mammalian cells.
- ChIP analysis showed that PNRC and RPC39 are co-recruited to RNA polymerase III-dependent gene promoters.
Takeaway
PNRC is a helper protein that makes another protein, RPC39, work better to create important molecules in our cells.
Methodology
Yeast two-hybrid screening, co-immunoprecipitation, and chromatin immunoprecipitation (ChIP) were used to study the interactions and effects of PNRC and RPC39.
Digital Object Identifier (DOI)
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