Complete Genome Sequence of Mycoplasma bovis Strain Hubei-1
Author Information
Author(s): Li Yuan, Zheng Huajun, Liu Yang, Jiang Yanwei, Xin Jiuqing, Chen Wei, Song Zhiqiang
Primary Institution: National Contagious Bovine Pleuropneumonia Reference Laboratory, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences
Hypothesis
Understanding the genetics and pathogenic mechanisms of Mycoplasma bovis would help differentiate between the genes, proteins, and nosogenesis of the two mycoplasms that cause contagious bovine pleuropneumonia and Mycoplasma bovis-associated diseases.
Conclusion
The genome of Mycoplasma bovis strain Hubei-1 was successfully sequenced, revealing important genetic features and potential virulence factors.
Supporting Evidence
- The genome of M. bovis strain Hubei-1 contains a single circular chromosome of 953,114 bp with a 29.37% GC content.
- 803 open reading frames (ORFs) were identified, with 34 ORFs being Hubei-1 specific.
- Genome analysis validated lateral gene transfer between M. bovis and Mycoplasma mycoides subspecies mycoides.
- 47 lipoproteins, 12 extracellular proteins, and 18 transmembrane proteins are phase-variable and may help M. bovis escape the immune response.
- Two possible pathogenicity islands were identified in the genome.
Takeaway
Scientists sequenced the DNA of a germ that makes cows sick to learn more about how it works and how to fight it.
Methodology
The complete genomic sequencing was conducted using a Roche GS FLX system, resulting in 38-fold genome coverage and assembly into 86 contigs.
Limitations
The study does not address the functional implications of all identified genes and potential interactions with host organisms.
Digital Object Identifier (DOI)
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