p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results
1994

p53 Protein Analysis in Breast Cancer

Sample size: 136 publication Evidence: moderate

Author Information

Author(s): J. Jacquemier, J.P. Moles, F. Penault-Llorca, J. Adelaide, M. Torrent, P. Viens, D. Bimbaum, C. Theillet

Primary Institution: Institut Paoli-Calmettes

Hypothesis

The study investigates the correlation between p53 protein expression and various prognostic factors in breast cancer.

Conclusion

The study found that p53 expression is associated with aggressive breast cancer characteristics but does not correlate with disease-free survival.

Supporting Evidence

  • p53 expression was detected in 38% of tumors with at least one antibody.
  • A statistically significant correlation was found between p53 expression and grade III disease.
  • No correlation was observed between p53 expression and lymph node involvement.

Takeaway

Researchers looked at a protein called p53 in breast cancer samples to see if it could help predict how aggressive the cancer is. They found that while p53 levels can indicate aggressive cancer, they don't help predict if the cancer will come back.

Methodology

The study analyzed p53 expression in 136 breast cancer samples using immunohistochemistry and PCR-SSCP for mutation detection.

Limitations

The follow-up period was short, limiting the ability to assess long-term outcomes.

Participant Demographics

Most participants were post-menopausal women, with a median follow-up of 10.7 months.

Statistical Information

P-Value

P<0.0001 for grade III disease correlation

Statistical Significance

p<0.05

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