Characterization of Human CD81 Protein Produced in Yeast
Author Information
Author(s): Jamshad Mohammed, Rajesh Sundaresan, Stamataki Zania, Jane A. McKeating, Timothy Dafforn, Michael Overduin, Roslyn M. Bill
Primary Institution: Aston University
Hypothesis
Can recombinant human CD81 be effectively produced and characterized in Pichia pastoris?
Conclusion
The study successfully produced and characterized recombinant human CD81, confirming its interaction with Hepatitis C virus E2 glycoprotein.
Supporting Evidence
- The purified CD81 protein was shown to interact with Hepatitis C virus E2 glycoprotein.
- Immunofluorescent staining confirmed the antigenicity of the recombinant CD81.
- Circular dichroism analysis indicated a high α-helical content in the protein structure.
- Analytical ultracentrifugation revealed that the recombinant CD81 is a single species in detergent solution.
Takeaway
Scientists made a special protein called CD81 in yeast, and they found out it can help the Hepatitis C virus get into cells.
Methodology
The study involved producing human CD81 in yeast, purifying it, and analyzing its structure using techniques like circular dichroism and analytical ultracentrifugation.
Limitations
The study does not provide insights into the full-length structure of CD81 or its interactions in a cellular context.
Digital Object Identifier (DOI)
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