Identification of two distinct structural regions in a human porcine endogenous retrovirus receptor, HuPAR2, contributing to function for viral entry
2009

Understanding HuPAR2's Role in Viral Entry

Sample size: 3 publication Evidence: high

Author Information

Author(s): Katherine T. Marcucci, Takele Argaw, Carolyn A. Wilson, Daniel R. Salomon

Primary Institution: The Scripps Research Institute

Hypothesis

What are the structural regions in HuPAR2 that contribute to its function for viral entry?

Conclusion

HuPAR2 has two distinct structural regions that influence its function for PERV-A entry.

Supporting Evidence

  • HuPAR2 is 11-fold more functional for PERV-A infection than HuPAR1.
  • The first 135 amino acids of HuPAR2 are critical for PERV-A entry.
  • Seven residues in HuPAR2 enhance its function without affecting envelope binding.

Takeaway

Scientists studied a protein called HuPAR2 to see how it helps a virus enter cells, finding two important parts that help it work better.

Methodology

The study involved creating chimeras and performing infection assays to assess receptor function.

Statistical Information

P-Value

p < 0.001

Statistical Significance

p < 0.001

Digital Object Identifier (DOI)

10.1186/1742-4690-6-3

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