Grape Seed Proanthocyanidins Inhibit Melanoma Cell Invasiveness by Reduction of PGE2 Synthesis and Reversal of Epithelial-to-Mesenchymal Transition
2011
Grape Seed Proanthocyanidins Inhibit Melanoma Cell Invasiveness
publication
Evidence: high
Author Information
Author(s): Mudit Vaid, Tripti Singh, Santosh K. Katiyar
Primary Institution: University of Alabama at Birmingham
Hypothesis
Do grape seed proanthocyanidins (GSPs) inhibit melanoma cell migration and invasion through the reduction of COX-2 expression and reversal of epithelial-to-mesenchymal transition?
Conclusion
Grape seed proanthocyanidins inhibit melanoma cell migration by reducing COX-2 expression and reversing the epithelial-to-mesenchymal transition.
Supporting Evidence
- GSPs reduced melanoma cell migration in a concentration-dependent manner.
- GSPs inhibited COX-2 expression and PGE2 production.
- GSPs reversed the epithelial-to-mesenchymal transition in melanoma cells.
- Treatment with celecoxib, a COX-2 inhibitor, also reduced melanoma cell migration.
- Transfection with COX-2 siRNA significantly decreased cell migration.
Takeaway
Grape seed extract can help stop skin cancer cells from moving around and spreading by changing how they behave.
Methodology
In vitro cell invasion assays were used to assess the effects of GSPs on melanoma cell migration and COX-2 expression.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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