Effects of Angiopoietins on Endothelial Cell Permeability
Author Information
Author(s): van der Heijden Melanie, van Nieuw Amerongen Geerten P., van Bezu Jan, Paul Marinus A., Groeneveld A. B. Johan, van Hinsbergh Victor W. M.
Primary Institution: VU University Medical Centre, Amsterdam, The Netherlands
Hypothesis
Ang-2 acts as a sensitizer of thrombin-induced hyperpermeability of human pulmonary microvascular endothelial cells, opposed by Ang-1.
Conclusion
Ang-1 reduces thrombin-induced permeability, while Ang-2 enhances it, particularly during the initial response.
Supporting Evidence
- Ang-2 enhanced the initial permeability increase in endothelial cells.
- Ang-1 preincubation reduced thrombin-induced permeability.
- The study used a model of human pulmonary microvascular endothelial cells.
Takeaway
This study shows that one protein (Ang-2) makes blood vessel walls leakier when they are activated, while another protein (Ang-1) helps keep them strong and less leaky.
Methodology
Permeability was assessed by measuring macromolecule passage and transendothelial electrical resistance (TEER) in cultured human pulmonary microvascular endothelial cells.
Limitations
The study's findings may not fully represent in vivo conditions due to the use of cultured cells.
Statistical Information
P-Value
0.010, 0.021, 0.027, 0.039
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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