Structural Organization of the 19S Proteasome Lid: Insights from MS of Intact Complexes
2006
Understanding the Structure of the 19S Proteasome Lid
publication
Evidence: moderate
Author Information
Author(s): Michal Sharon, Carol Robinson
Hypothesis
How does the structural organization of the 19S proteasome lid contribute to its function?
Conclusion
The study reveals the structural organization of the 19S proteasome lid and identifies key protein interactions.
Supporting Evidence
- The mass spectrum confirmed the lid consists of nine protein components.
- The intact complex was identified as roughly 376 kiloDaltons.
- Tandem mass spectrometry revealed interactions between proteins Rpn6, Rpn9, and Rpn12.
- Chemical cross-linking identified additional interaction partners within the lid.
Takeaway
The researchers figured out how the parts of a protein complex fit together, which helps us understand how it works.
Methodology
The study used mass spectrometry and chemical cross-linking to analyze the structure of the 19S proteasome lid.
Digital Object Identifier (DOI)
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