Study of Drug Resistance in Malaria Parasites in India
Author Information
Author(s): Prajapati Surendra K, Joshi Hema, Dev Vas, Dua Virendra K
Primary Institution: National Institute of Malaria Research (NIMR)
Hypothesis
This study aims to identify point mutations in dhfr/dhps and the spatio-temporal pattern of anti-folate drug resistance in the Indian subcontinent.
Conclusion
The study suggests that P. vivax may be susceptible to anti-folate drugs in India, except in the Andaman and northeastern states.
Supporting Evidence
- The study identified several point mutations in the dhfr and dhps genes associated with drug resistance.
- A significant increase in the frequency of quadruple mutant dhfr genotype was observed in the Kamrup region over five years.
- Geographical clustering of dhfr genotypes was identified, with distinct patterns of resistance in different regions.
Takeaway
The study looked at how malaria parasites in India are becoming resistant to certain drugs, and found that some areas have more resistant parasites than others.
Methodology
Blood samples from 100 P. vivax field isolates were collected from five regions in India, and dhfr and dhps genes were PCR amplified and sequenced.
Potential Biases
Potential bias in sampling due to geographical clustering and selection of isolates.
Limitations
The study may not cover all geographical regions of India, and the sample size from some areas was limited.
Participant Demographics
Participants were symptomatic patients from five widely separated geographical regions of India.
Statistical Information
P-Value
<0.0001
Confidence Interval
95% CI = ± 4.9
Statistical Significance
p < 0.001
Digital Object Identifier (DOI)
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