Complete shutdown of microvascular perfusion upon hepatic cryothermia is critically dependent on local tissue temperature
2000
Impact of Temperature on Liver Cryosurgery
publication
Author Information
Author(s): Schüder G, Pistorius G, Fehringer M, Feifel G, Menger M D, Vollmar B
Primary Institution: University of Saarland
Hypothesis
What temperature is necessary to induce complete nutritive perfusion failure in cryothermia-treated rat livers?
Conclusion
Freezing liver tissue to temperatures below 0°C causes complete and irreversible perfusion failure, leading to cell death and tissue necrosis.
Supporting Evidence
- Cryothermia resulted in a central non-perfused part of injury, surrounded by a heterogeneously perfused peripheral zone.
- The non-perfused area after single and double freezing continuously increased over the first 90 minutes.
- The critical border temperature for perfusion failure was found to be between 8.29°C and 9.07°C.
Takeaway
If you freeze liver tissue too much, it stops getting blood, which can kill the cells.
Methodology
The study used in vivo fluorescence microscopy and thermocouples to measure tissue temperature during cryotherapy in rat livers.
Statistical Information
Confidence Interval
8.29 ± 1.63°C and 9.07 ± 0.24°C
Digital Object Identifier (DOI)
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