Biochemical Features of Movement Protein of RDV
2011
Movement Protein Pns6 of Rice Dwarf Phytoreovirus Binds RNA and Has ATPase Activity
publication
Evidence: moderate
Author Information
Author(s): Ji Xu, Qian Dan, Wei Chunhong, Ye Gongyin, Zhang Zhongkai, Wu Zujian, Xie Lianhui, Li Yi
Primary Institution: State Key Laboratory of Protein and Plant Gene Research, Peking University
Hypothesis
Does the movement protein Pns6 of Rice dwarf phytoreovirus interact with genomic dsRNAs or their derivative sRNAs?
Conclusion
Pns6 binds both dsRNAs and ssRNAs, showing preference for ssRNA sequences derived from the conserved genomic terminal consensus sequences of RDV.
Supporting Evidence
- Pns6 exhibits non-sequence specificity for dsRNA but shows preference for ssRNA sequences.
- Pns6 has magnesium-dependent ATPase activities.
- Mutagenesis identified the RNA binding and ATPase activity sites of Pns6.
Takeaway
The protein Pns6 helps the Rice dwarf virus move between plant cells by binding to RNA and using energy from ATP.
Methodology
The study involved biochemical assays to analyze the RNA binding and ATPase activities of the Pns6 protein.
Digital Object Identifier (DOI)
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