Reducing Orc6 Makes Colon Cancer Cells More Sensitive to Chemotherapy
Author Information
Author(s): Gavin Elaine J., Song Bo, Wang Yuan, Xi Yaguang, Ju Jingfang
Primary Institution: University of South Alabama, Mobile, Alabama, United States of America
Hypothesis
Orc6 may be a key player that is involved in colorectal cancer and its elevated level may contribute to chemoresistance.
Conclusion
Down regulation of Orc6 in colon cancer cells sensitizes them to 5-FU and cisplatin treatment.
Supporting Evidence
- Orc6 expression was found to be highly elevated in colorectal cancer patient specimens.
- Decreasing Orc6 expression triggered cell cycle arrest at G1 phase in colon cancer cells.
- HCT-116 (wt-p53) cells with reduced Orc6 expression were 5-fold more sensitive to 5-FU treatment compared to control cells.
Takeaway
When scientists reduced the Orc6 protein in colon cancer cells, those cells became more sensitive to chemotherapy drugs, which could help in treating cancer better.
Methodology
The study used HCT116 colon cancer cell lines with different p53 statuses and employed siRNA to reduce Orc6 expression, followed by treatment with 5-FU and cisplatin.
Limitations
The study primarily focused on two specific colon cancer cell lines and may not represent all colorectal cancers.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website