Gene Expression Variability in Mice
Author Information
Author(s): Colin Pritchard, David Coil, Sarah Hawley, Li Hsu, Peter S. Nelson
Primary Institution: Fred Hutchinson Cancer Research Center
Hypothesis
What are the contributions of normal variation and genetic background to mammalian gene expression?
Conclusion
The study found that significant gene expression variability exists among genetically identical mice, suggesting that factors beyond DNA sequence influence gene expression.
Supporting Evidence
- 23% to 44% of all genes exhibited differences in expression levels between genetically identical individuals.
- Approximately 3% of all genes exhibited inter-strain expression differences.
- Genes associated with cell growth, cytokine activity, amine metabolism, and ubiquitination were enriched among those with significant variability.
Takeaway
Even mice that are genetically the same can have different levels of gene activity, which can affect how they look and behave.
Methodology
The study used microarray analysis to measure gene expression levels in the livers of five different mouse strains.
Potential Biases
Potential technical inconsistencies in RNA isolation and cDNA synthesis could introduce bias.
Limitations
The study may not account for all environmental factors influencing gene expression variability.
Participant Demographics
The study involved male mice from five different strains: 129, Balb/c, CD1, FVB, and CFW.
Statistical Information
P-Value
p<0.00002
Statistical Significance
p<0.00002
Digital Object Identifier (DOI)
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