Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expression
2011
MATR3 and HIV Rev: Understanding mRNA Regulation
Commentary
Author Information
Author(s): Andrew Dayton
Primary Institution: FDA/CBER/OBRR/DETTD/LMV
Hypothesis
MATR3 acts as a co-factor for HIV-1 Rev in facilitating the export and translation of viral mRNA.
Conclusion
MATR3 is essential for the cytoplasmic accumulation and translation of unspliced HIV mRNA.
Supporting Evidence
- MATR3 binds intracellularly to HIV mRNA.
- MATR3 has no direct effect on transcription from the HIV LTR.
- Rev needs MATR3 to promote the cytoplasmic accumulation of unspliced mRNA.
- Rev, MATR3, and RRE-containing mRNA form a detectable intracellular complex.
Takeaway
MATR3 helps HIV's Rev protein move its genetic material out of the cell's nucleus so it can make more viruses.
Methodology
The study involved MS2 coat protein recognition sequence tags and MS2 pull down to identify host proteins bound to HIV mRNA.
Digital Object Identifier (DOI)
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