Proteomic Analysis of the Secretory Response of Aspergillus niger to D-Maltose and D-Xylose
Author Information
Author(s): José Miguel Ferreira de Oliveira, Mark W. J. van Passel, Peter J. Schaap, Leo H. de Graaff
Primary Institution: Wageningen University, Wageningen, The Netherlands
Hypothesis
This study aims to investigate the changes in secretome and microsomal protein composition of Aspergillus niger induced by D-maltose and D-xylose.
Conclusion
The study confirms that D-maltose and D-xylose each induce the production of specific extracellular enzymes in Aspergillus niger.
Supporting Evidence
- 102 secreted proteins and 1,126 microsomal proteins were identified in this study.
- Induction by D-maltose or D-xylose resulted in the increase in specific extracellular enzymes.
- Millimolar amounts of the inducers D-maltose and D-xylose are sufficient to cause a direct response in specific protein expression levels.
Takeaway
Scientists studied how a fungus called Aspergillus niger makes special proteins when it eats different sugars, finding that two sugars make it produce different proteins.
Methodology
A proteomics approach was used to analyze the changes in the secretome and microsomal proteome of A. niger after induction of extracellular enzyme production.
Limitations
The study did not analyze the total intracellular proteome of A. niger, which may limit the understanding of protein release mechanisms.
Statistical Information
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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