Expression profiling of all protein-coding genes in wild-type and three DNA repair-deficient substrains of Escherichia coli K-12

2002

Gene Expression in E. coli Strains with DNA Repair Deficiencies

Sample size: 4 publication 10 minutes Evidence: moderate

Author Information

Author(s): Camilla Salmelin, Juhani Vilpo

Primary Institution: Leukemia Research Laboratory, Department of Clinical Chemistry, Tampere University Hospital and Tampere University Medical School

Hypothesis

Does the disruption of a single gene in E. coli lead to significant changes in the expression of other genes?

Conclusion

Disruption of a single gene does not necessarily result in significant changes in the expression of other genes in E. coli under good growth conditions.

Supporting Evidence

81 deviations of the expression of 59 genes were noted out of 12,870 when 3-fold or greater up- or down-regulation was used as a criterion.
The expression profiles of the four E. coli strains were very similar.
Only 0.46% of the genes in the three mutants showed a 3-fold or greater difference compared with the wild-type.

Takeaway

Scientists studied how changing one gene in E. coli affects other genes. They found that changing one gene doesn't always change how other genes work.