Using Archived Tissue Samples for Genetic Analysis
Author Information
Author(s): Cannon-Albright Lisa A, Cooper Kendal G, Georgelas Ann, Bernard Philip S
Primary Institution: University of Utah School of Medicine
Hypothesis
DNA could be extracted from normal tissue found in most FFPE samples, and that this would provide adequate genome-wide germline DNA for genotyping.
Conclusion
DNA extracted from normal FFPE tissue provides excellent quality and quantity genome-wide genotyping data representing germline DNA.
Supporting Evidence
- Four of the five FFPE samples had identifiable normal tissue.
- Concordance rates of over 99% were observed for samples with DNA from both FFPE and whole blood.
- Genotyping call rates for the 4 DNA case samples from FFPE ranged from 0.873 to 0.989.
Takeaway
Scientists found that they can get good DNA from old tissue samples stored in a special way, which helps them study genetics even when people can't give new samples.
Methodology
DNA was extracted from normal tissue in FFPE samples and analyzed for genome-wide genotyping.
Limitations
The integrity of the DNA was questionable due to significant fragmentation.
Participant Demographics
Prostate cancer cases with FFPE tissue samples stored between 18-41 years.
Digital Object Identifier (DOI)
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