Plasma-Activated Medium and Lung Cancer Cells
Author Information
Author(s): Sun Zhidan, Ding Chenglong, Wang Yuhan, Lu Tingting, Song Wencheng
Primary Institution: Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences
Hypothesis
Can plasma-activated medium inhibit the proliferation and migration of non-small cell lung cancer A549 cells in 3D culture?
Conclusion
Plasma-activated medium effectively inhibits the proliferation, migration, and invasion of A549 cells in both 2D and 3D cultures.
Supporting Evidence
- PAM treatment inhibited the viability, migration, and invasion abilities of A549 cells in both 2D and 3D cultures.
- PAM suppressed the epithelial–mesenchymal transition process.
- PAM downregulated the expression of the RAS/ERK signaling pathway.
- PAM treatment exhibited greater cytotoxicity in 2D culture compared to 3D culture.
- ROS-induced oxidative reactions mediated the effects of PAM on A549 cells.
Takeaway
Researchers found that a special treatment called plasma-activated medium can stop lung cancer cells from growing and moving around, which is important for fighting cancer.
Methodology
The study used MTT, migration, invasion assays, and Western Blot analysis to assess the effects of PAM on A549 cells in 2D and 3D cultures.
Limitations
The study was limited by the inability to test the effects of chemotherapy drugs in monotherapy and combination therapies.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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