The Inhibitory Effect of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) on the Monophenolase and Diphenolase Activities of Mushroom Tyrosinase
2011
Effects of NSAIDs on Mushroom Tyrosinase Activity
publication
Evidence: moderate
Author Information
Author(s): Sato Kazuomi, Toriyama Masaru
Primary Institution: Tamagawa University
Hypothesis
The study investigates the inhibitory effects of various NSAIDs on the activities of mushroom tyrosinase.
Conclusion
Diflunisal and indomethacin significantly inhibit both monophenolase and diphenolase activities of mushroom tyrosinase.
Supporting Evidence
- Diflunisal inhibited monophenolase activity with an IC50 of 0.112 mM.
- Indomethacin inhibited monophenolase activity with an IC50 of 1.78 mM.
- Diflunisal inhibited diphenolase activity with an IC50 of 0.197 mM.
- Indomethacin inhibited diphenolase activity with an IC50 of 0.509 mM.
Takeaway
Some common pain relievers can stop a specific enzyme in mushrooms that helps with skin color and browning in fruits and vegetables.
Methodology
The study used tyrosinase assays to measure the effects of NSAIDs on monophenolase and diphenolase activities using l-tyrosine and l-DOPA as substrates.
Limitations
The study only tested a limited number of NSAIDs and did not explore their long-term effects.
Digital Object Identifier (DOI)
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