BMP-2 Gene-Fibronectin-Apatite Composite Layer Enhances Bone Formation
Author Information
Author(s): Zhang Wei, Tsurushima Hideo, Oyane Ayako, Yazaki Yushin, Sogo Yu, Ito Atsuo, Matsumura Akira
Primary Institution: Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST)
Hypothesis
Can an apatite composite layer including BMP-2 gene and fibronectin enhance bone formation?
Conclusion
The BMP-2 gene-FB-apatite composite layer might be useful for bone engineering.
Supporting Evidence
- The apatite composite layer provided more efficient gene transfer for the cultured cells than an apatite composite layer without FB.
- Bone formation was more enhanced in the HAP-BMP-FB group than in the control groups.
- The BMP-2 concentration was approximately 100~600 pg/mL in the cell-culture medium.
- ALP activity and OC concentrations increased when cells were cultured on the apatite composite layer.
Takeaway
This study shows that a special layer with a gene can help bones grow better, which is important for healing.
Methodology
Cells were cultured on a composite layer and gene expression, protein concentrations, and bone formation were measured in both in vitro and in vivo experiments.
Potential Biases
Potential bias in animal model selection and small sample sizes.
Limitations
The study primarily focused on short-term effects and did not evaluate long-term bone healing.
Participant Demographics
Seven- to eight-week-old male Wistar rats were used in the animal experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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