Identification, cloning and functional characterization of novel sperm associated antigen 11 (SPAG11) isoforms in the rat
2006
Characterization of Novel Sperm Associated Antigen Isoforms in Rats
Sample size: 15
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Yenugu Suresh, Hamil Katherine G, Grossman Gail, Petrusz Peter, French Frank S, Hall Susan H
Primary Institution: University of North Carolina, Chapel Hill, NC, USA
Hypothesis
The expression of Spag11 transcripts in rodents is not well documented.
Conclusion
The abundant expression of Spag11t and Spag11c in the male reproductive tract suggests an important role in male reproductive tract immunity.
Supporting Evidence
- Two novel Spag11 transcripts, Spag11t and Spag11c, were identified in the rat.
- Spag11c is expressed in the epididymis, testis, and ovary, while Spag11t is confined to the caput epididymis.
- Both isoforms showed age-dependent expression, peaking in adult rats.
Takeaway
This study found new proteins in rats that help protect sperm and are important for their development.
Methodology
Computational analysis and RT-PCR were used to identify and characterize Spag11 isoforms in rat reproductive tissues.
Participant Demographics
Wistar rats aged 10 to 60 days and adult Sprague-Dawley rats.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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