Study of β-lactamases in Yersinia intermedia and Y. frederiksenii
Author Information
Author(s): Mittal Shilpi, Mallik Sarita, Sharma Sachin, Virdi Jugsharan S
Primary Institution: Microbial Pathogenicity Laboratory, Department of Microbiology, University of Delhi South Campus
Hypothesis
The objective of this work was to study the characteristics of β-lactamases and their genes in strains of Y. intermedia and Y. frederiksenii isolated from clinical and non-clinical sources in India.
Conclusion
Both Y. intermedia and Y. frederiksenii showed presence of blaA and blaB genes and unequivocal expression of the two β-lactamases.
Supporting Evidence
- The enzymes Bla-A and Bla-B were found in all clinical and non-clinical strains.
- Differential expression of Bla-A was observed, while Bla-B showed consistent expression.
- The presence of these enzymes was reflected in high minimum inhibitory concentrations of β-lactam antibiotics.
Takeaway
The study found that two types of enzymes, Bla-A and Bla-B, are present in certain bacteria, which can make them resistant to antibiotics.
Methodology
The study used double disc diffusion method, minimum inhibitory concentration (MIC) testing, PCR amplification, and restriction fragment length polymorphism (RFLP) to analyze the presence and characteristics of β-lactamases.
Limitations
The study primarily focused on strains isolated from India, which may limit the generalizability of the findings.
Participant Demographics
Strains were isolated from clinical (diarrheic human subjects) and non-clinical sources (pig throat and wastewater) in India.
Digital Object Identifier (DOI)
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