The 3-Base Periodicity and Codon Usage of Coding Sequences Are Correlated with Gene Expression at the Level of Transcription Elongation Codon Frequencies and Transcription Rate
2011

Correlation of 3-Base Periodicity and Gene Expression in Yeast

Sample size: 408 publication 10 minutes Evidence: high

Author Information

Author(s): Edoardo Trotta, Grzegorz Kudla

Primary Institution: Institute of Translational Pharmacology, Consiglio Nazionale delle Ricerche, Roma, Italy

Hypothesis

Does the 3-base periodicity of coding sequences correlate with gene expression levels in yeast?

Conclusion

The study shows that the 3-base periodicity of coding sequences is correlated with gene expression, particularly at the transcription elongation stage.

Supporting Evidence

  • The analysis identified that codon frequency accounts for 71.9% of the variability in mRNA levels.
  • Codon usage and the relative abundance of tRNA can drive each other to optimize gene expression efficiency.
  • The study proposes that selection on codon bias acts at both transcriptional and translational levels.

Takeaway

This study found that the way DNA is structured can affect how much of a gene is made into proteins, especially in yeast.

Methodology

The study analyzed the 3-base periodicity of coding sequences and its correlation with gene expression using various statistical models and indices.

Limitations

The study primarily focuses on yeast and may not be generalizable to other organisms.

Participant Demographics

The study analyzed coding sequences from Saccharomyces cerevisiae.

Statistical Information

P-Value

p<0.0001

Statistical Significance

p<0.0001

Digital Object Identifier (DOI)

10.1371/journal.pone.0021590

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