Study of Duck Enteritis Virus UL53 Gene and Glycoprotein K
Author Information
Author(s): Zhang Shunchuan, Xiang Jun, Cheng Anchun, Wang Mingshu, Wu Ying, Yang Xiaoyuan, Zhu Dekang, Jia Renyong, Luo Qihui, Chen Zhengli, Chen Xiaoyue
Primary Institution: Sichuan Agricultural University
Hypothesis
What are the transcription characteristics and intracellular localization of the UL53 gene and its product gK in Duck Enteritis Virus?
Conclusion
The DEV UL53 gene is identified as a really late gene, and its product gK is mainly localized in the cytoplasm.
Supporting Evidence
- The UL53 gene was first transcribed in DEF cells at 10 hours post-infection.
- The gK protein was detected in infected cells starting at 12 hours post-infection.
- The intracellular localization of gK was primarily in the cytoplasm.
Takeaway
This study looks at a virus that affects ducks and finds that a specific gene is important for how the virus works and where its proteins are found in the cells.
Methodology
Fluorescent quantitative real-time PCR (FQ-RT-PCR), nucleic acid inhibition test, Western blotting, and indirect immunofluorescence assays were used to study the UL53 gene and gK protein.
Digital Object Identifier (DOI)
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