A study on trypsin, Aspergillus flavus and Bacillus sp. protease inhibitory activity in Cassia tora (L.) syn Senna tora (L.) Roxb. seed extract
2011

Protease Inhibitory Activity of Cassia Tora Seed Extract

publication Evidence: high

Author Information

Author(s): Vinayak R Tripathi, Shailendra Kumar, Satyendra K Garg

Primary Institution: Centre of Excellence in Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh, India

Hypothesis

The study investigates the protease inhibitory activity of Cassia tora seed extract against trypsin, Aspergillus flavus, and Bacillus sp. proteases.

Conclusion

Cassia tora seed extract has strong protease inhibitory activity against trypsin, Aspergillus flavus, and Bacillus sp. proteases.

Supporting Evidence

  • The crude seeds' extract displayed strong antitryptic, bacterial and fungal protease inhibitory activity.
  • The seed protein fraction 30-60% was found most active for trypsin inhibition.
  • The inhibition of caseinolytic activity of the proteases increased with increasing ratio of seed extract.

Takeaway

The seeds of Cassia tora can help stop certain bad germs from working by blocking their proteins.

Methodology

The study involved extracting proteins from Cassia tora seeds and testing their inhibitory effects on various proteases using spot tests and caseinolytic assays.

Statistical Information

P-Value

< 0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1186/1472-6882-11-56

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