Vesnarinone, a differentiation inducing drug, directly activates p21waf1 gene promoter via Sp1 sites in a human salivary gland cancer cell line
2002
Vesnarinone Activates p21waf1 in Salivary Gland Cancer Cells
publication
Evidence: moderate
Author Information
Author(s): F Omotehara, H Kawamata, D Uchida, S Hino, K Nakashiro, T Fujimori
Primary Institution: Dokkyo University School of Medicine
Hypothesis
How does vesnarinone induce the expression of the p21waf1 gene in a human salivary gland cancer cell line?
Conclusion
Vesnarinone directly activates the p21waf1 promoter through Sp1 and Sp3 transcription factors and induces histone hyperacetylation in TYS cells.
Supporting Evidence
- Vesnarinone enhances luciferase activity from the p21waf1 promoter in TYS cells.
- The minimal vesnarinone-responsive element in the p21waf1 promoter was identified.
- Sp1 and Sp3 transcription factors bind to the vesnarinone-responsive element.
Takeaway
Vesnarinone is a drug that helps stop cancer cells from growing by turning on a gene that slows down the cell cycle.
Methodology
The study used luciferase reporter assays and electrophoretic mobility shift assays to investigate the activation of the p21waf1 promoter by vesnarinone.
Digital Object Identifier (DOI)
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