Understanding CXCL12 Signaling in T Cells
Author Information
Author(s): Wojcechowskyj Jason A., Lee Jessica Y., Seeholzer Steven H., Doms Robert W.
Primary Institution: University of Pennsylvania School of Medicine
Hypothesis
How does CXCL12 signaling affect T cell function and potential therapeutic targets?
Conclusion
The study identified 89 phosphopeptides that respond to CXCL12 signaling in T cells, providing insights into potential therapeutic targets for cancer and HIV.
Supporting Evidence
- 89 phosphopeptides were identified as CXCL12-responsive.
- Pathway analysis revealed enrichment for T cell activation and mTOR signaling.
- Several novel phosphosites were validated by Western blot.
Takeaway
This study looked at how a special signal called CXCL12 affects T cells, which are important for our immune system, and found new ways to help treat diseases like cancer and HIV.
Methodology
The researchers used mass spectrometry-based phosphoproteomics and stable isotope labeling with amino acids in cell culture (SILAC) to analyze CXCL12 signaling in human T cells.
Potential Biases
Potential biases may arise from the specific cell line used and the experimental conditions.
Limitations
The study focused on a single time point and cell type, which may limit the generalizability of the findings.
Participant Demographics
Human lymphoblastic CEM cell line was used for the study.
Statistical Information
P-Value
p=3.2×10−5
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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