Multiplex PCR Assay for Salmonella Typhimurium
Author Information
Author(s): Marie Bugarel, Sophie Granier, François-Xavier Weill, Patrick Fach, Anne Brisabois
Primary Institution: French Agency for Food, Environmental and Occupational Health Safety (ANSES)
Hypothesis
This study aimed to detect the prevalence of ten markers combined in a macro-array based on multiplex real-time PCR.
Conclusion
The GeneDisc® assay developed in this study made it easier to explore variability within serotype Typhimurium by analyzing ten relevant gene determinants in a large collection of strains.
Supporting Evidence
- 34 different genotypes were identified among the 538 strains.
- Three major genotypes were encountered in 75% of the studied strains.
- SPI determinants were almost always detected in the strains.
Takeaway
The researchers created a new test to quickly check for important genes in Salmonella Typhimurium, helping to understand how different strains vary.
Methodology
The study used a GeneDisc® array to simultaneously detect 10 specific gene targets in Salmonella Typhimurium strains.
Potential Biases
The selection of strains was not evenly distributed across sources, which may introduce bias.
Limitations
The study may have bias due to the voluntary nature of strain collection and uneven distribution of tested strains from different sources.
Participant Demographics
Strains were isolated from various sources including food animals, food products, human, and environmental samples.
Statistical Information
Confidence Interval
95%
Digital Object Identifier (DOI)
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