A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities
2007
A multipurpose biocatalyst with pectinase, xylanase, and cellulase activities
publication
Evidence: high
Author Information
Author(s): Dalal Sohel, Sharma Aparna, Gupta Munishwar Nath
Primary Institution: Chemistry Department, Indian Institute of Technology Delhi
Hypothesis
Can a single biocatalyst be designed to perform multiple enzymatic reactions?
Conclusion
The study successfully developed a robust biocatalyst that is more stable and reusable for hydrolyzing pectin, xylan, and cellulose.
Supporting Evidence
- The CLEA retained 100% of enzyme activities after cross-linking.
- The half-lives of the enzymes increased significantly after immobilization.
- The CLEA could be reused three times without loss of activity.
Takeaway
Scientists made a special enzyme that can help break down different types of plant materials, and it can be used many times without losing its power.
Methodology
The biocatalyst was prepared by precipitating enzyme activities with n-propanol and cross-linking with glutaraldehyde.
Digital Object Identifier (DOI)
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