Nicotine Acts on Growth Plate Chondrocytes to Delay Skeletal Growth through the α7 Neuronal Nicotinic Acetylcholine Receptor
2008

Nicotine Delays Bone Growth

publication Evidence: moderate

Author Information

Author(s): Kawakita Atsuo, Sato Kazuki, Makino Hatsune, Ikegami Hiroyasu, Takayama Shinichiro, Toyama Yoshiaki, Umezawa Akihiro

Primary Institution: National Institute for Child Health and Development, Tokyo, Japan

Hypothesis

Nicotine affects growth plate chondrocytes directly and specifically through nAChR to delay skeletal growth.

Conclusion

Nicotine acts directly on growth plate chondrocytes to decrease matrix synthesis and suppress hypertrophic differentiation, leading to delayed skeletal growth.

Supporting Evidence

  • Nicotine inhibited matrix synthesis and hypertrophic differentiation in human growth plate chondrocytes.
  • Maternal nicotine exposure resulted in delayed skeletal growth of alpha7 nAChR +/+ fetuses.
  • The effect of nicotine on chondrocytes was reversed by MLA, a specific antagonist of alpha7 nAChR.

Takeaway

Nicotine from smoking can slow down how bones grow in children by affecting special cells that help bones grow.

Methodology

The study investigated the effect of nicotine on human growth plate chondrocytes derived from extra human fingers, using both in vitro and in vivo experiments.

Limitations

The study primarily used chondrocytes from human fingers, which may not fully represent all growth plate chondrocytes.

Participant Demographics

Chondrocytes were derived from extra human fingers of patients with polydactyly.

Statistical Information

P-Value

p<0.02

Statistical Significance

p<0.02

Digital Object Identifier (DOI)

10.1371/journal.pone.0003945

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