AKAP12 and AKAP5 form higher-order hetero-oligomers
2011
AKAP5 and AKAP12 form higher-order hetero-oligomers
publication
Evidence: moderate
Author Information
Author(s): Gao Shujuan, Wang Hsien-yu, Malbon Craig C
Primary Institution: State University of New York at Stony Brook
Hypothesis
Whether AKAP5 and AKAP12 can hetero-oligomerize into supermolecular scaffolds of increased complexity is unknown.
Conclusion
AKAP5 and AKAP12 are capable of forming hetero-oligomeric supermolecular complexes that influence AKAP locale and function.
Supporting Evidence
- AKAP5 and AKAP12 were shown to form minimally hetero-dimers.
- The presence of AKAP12 increased the docking of AKAP5 by 4-fold upon stimulation.
- Steric-exclusion chromatography revealed large supermolecular complexes containing both AKAPs.
Takeaway
This study shows that two proteins, AKAP5 and AKAP12, can stick together in a new way, which helps them work better in sending signals inside cells.
Methodology
Affinity chromatography and steric-exclusion chromatography were used to analyze the interactions and oligomerization of AKAP5 and AKAP12.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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