Real-time Monitoring of Phage Suspensions for Personalized Therapy
Author Information
Author(s): Lapras Benjamine, Merienne Camille, Eynaud Emma, Usseglio Léa, Marchand Chloé, Médina Mathieu, Kolenda Camille, Briot Thomas, Laurent Frédéric, Pirot Fabrice
Primary Institution: Hospices Civils de Lyon
Hypothesis
Can interferometric light microscopy (ILM) effectively monitor phage suspensions in real-time for personalized phage therapy?
Conclusion
ILM can accurately approximate the infectious titre of phage suspensions and is a valuable tool for monitoring purification processes.
Supporting Evidence
- ILM showed a linear correlation between particle concentration and phage infectious titre.
- Purification processes resulted in a narrowing of size distribution and fewer aggregates.
- ILM was more effective than other methods in detecting aggregates.
Takeaway
This study shows that a special microscope can quickly check how many viruses are in a sample, which helps doctors make better treatments for infections.
Methodology
Interferometric light microscopy was used to measure particle concentration and size distribution of phage suspensions at various purification steps.
Potential Biases
Potential bias due to the inability of titration to detect all active particles.
Limitations
ILM cannot detect podoviruses or measure particles in low-concentrated suspensions.
Statistical Information
P-Value
p<0.001
Confidence Interval
95%
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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