Plasmon-Enhanced Fluorescence of Single Extracellular Vesicles
Author Information
Author(s): Yang Yupeng, Metem Prattakorn, Khaksaran Mohammad Hadi, Sahu Siddharth Sourabh, Stridfeldt Fredrik, Görgens André, Zhang Shi-Li, Dev Apurba
Primary Institution: Uppsala University
Hypothesis
Can a plasmonic nanohole array enhance the fluorescence detection of single extracellular vesicles?
Conclusion
The plasmonic nanohole array significantly increases the detection of weakly fluorescent extracellular vesicles.
Supporting Evidence
- The plasmonic nanohole array design allows for exclusive detection of single EVs.
- Fluorescence enhancement was estimated by measuring CD9 expression of small EVs.
- The number of observed EVs increased by more than 12 times compared to a reference substrate.
- Fluorescence intensities showed a moderate overall enhancement.
- Simulation and experimental results showed good agreement.
Takeaway
Scientists created a special chip that helps them see tiny particles called extracellular vesicles better by making their glow brighter.
Methodology
The study used a plasmonic nanohole array to capture single extracellular vesicles and measure their fluorescence using various microscopy techniques.
Limitations
The study may not account for all variables affecting fluorescence enhancement, such as the heterogeneity of protein expression on EVs.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website