Comparison of Two HPV DNA Tests
Author Information
Author(s): Diane C. Halstead, Sharon L.H. Pfleger, William Dupree
Primary Institution: Lehigh Valley Hospital
Hypothesis
This study was designed to compare the sensitivity, specificity, efficiency, positive and negative predictive values, and ease of use for two commercially available hybridization kits for detecting human papillomavirus (HPV) DNA.
Conclusion
Although SB has been considered the standard model, DB is an acceptable method for detecting and identifying HPV infections.
Supporting Evidence
- 106 out of 109 results were concordant between the two tests.
- The sensitivity of the ViraType DB was found to be 100% when compared to the Oncor SB.
- Only the number of sexual partners was a significant risk factor for predicting HPV infection.
Takeaway
The study looked at two tests for HPV and found that one test works just as well as the other for finding the virus.
Methodology
A total of 179 specimens were assessed for acceptability and tested for HPV by two methods: Oncor Southern blot and Digene ViraType dot blot.
Limitations
The study had a limited number of biopsy specimens and may not represent all patient populations.
Participant Demographics
The study included 172 cervical specimens from females aged 13 to 77 years and 7 penile biopsies.
Statistical Information
P-Value
0.000
Statistical Significance
p<0.05
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