MMP28 Expression in Intervertebral Discs and Its Response to Inflammation
Author Information
Author(s): Klawitter Marina, Quero Lilian, Bertolo Alessando, Mehr Marco, Stoyanov Jivko, Nerlich Andreas G, Klasen Juergen, Aebli Nikolaus, Boos Norbert, Wuertz Karin
Primary Institution: University of Zurich
Hypothesis
Increased expression of MMP28 correlates with higher grades of degeneration and is stimulated by the presence of proinflammatory molecules.
Conclusion
MMP28 expression in intervertebral discs is not regulated by inflammatory mechanisms and does not correlate with the grade of degeneration.
Supporting Evidence
- MMP28 expression was increased in individual cases with trauma or disc degeneration.
- There was no significant correlation between the grade of disease and MMP28 expression.
- Stimulation with inflammatory mediators did not alter MMP28 gene expression.
Takeaway
The study found that a protein called MMP28 in back discs doesn't change with inflammation or the level of damage to the discs.
Methodology
Gene expression levels of MMP28 were investigated in human intervertebral disc tissue and isolated cells after stimulation with inflammatory mediators using real-time RT PCR.
Limitations
High donor-donor variation in MMP28 expression and lack of statistical correlation with degeneration grades.
Participant Demographics
Thirteen tissue samples from eight patients diagnosed with symptomatic degenerative disc disease or spinal trauma.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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