How Lipopolysaccharide Affects the P2X7 Receptor Activity
Author Information
Author(s): Elias Leiva-Salcedo, Claudio Coddou, Felipe E. Rodríguez, Antonello Penna, Ximena Lopez, Tanya Neira, Ricardo Fernández, Mónica Imarai, Miguel Rios, Jorge Escobar, Margarita Montoya, J. Pablo Huidobro-Toro, Alejandro Escobar, Claudio Acuña-Castillo
Primary Institution: Centro Fondap de Estudios Moleculares de la Célula, Universidad de Chile
Hypothesis
Does lipopolysaccharide (LPS) modulate the activity of the P2X7 receptor?
Conclusion
LPS inhibits the activity of the P2X7 receptor, affecting calcium influx and apoptosis in T-regulatory cells.
Supporting Evidence
- LPS alone does not activate the P2X7 receptor but inhibits ATP-induced currents.
- LPS treatment protects T-regulatory cells from ATP-induced apoptosis.
- LPS modulates the activity of the P2X7 receptor without affecting ERK activation.
Takeaway
This study found that a substance from bacteria called LPS can stop a specific receptor in our cells from working properly, which might help protect certain immune cells from dying.
Methodology
The study used HEK293 cells to test the effects of LPS on P2X7 receptor activity, measuring ATP-induced currents, calcium influx, and apoptosis.
Limitations
The study primarily used a cell line and may not fully represent in vivo conditions.
Participant Demographics
Male and female 6–8-week-old C57BL/6 mice were used for splenocyte extraction.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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