FXR and Apoptosis in Barrett's Esophagus
Author Information
Author(s): De Gottardi Andrea, Dumonceau Jean-Marc, Bruttin Fabien, Vonlaufen Alain, Morard Isabelle, Spahr Laurent, Rubbia-Brandt Laura, Frossard Jean-Louis, Dinjens Winand NM, Rabinovitch Peter S, Hadengue Antoine
Primary Institution: University Hospital, Geneva, Switzerland
Hypothesis
FXR and VDR are expressed in the esophagus and may contribute to the regulation of apoptosis in intestinal metaplasia.
Conclusion
The bile acid receptor FXR is significantly overexpressed in Barrett's esophagus compared to normal mucosa, and guggulsterone treatment enhances apoptosis in Barrett's esophagus-derived cells.
Supporting Evidence
- FXR expression was significantly higher in Barrett's esophagus compared to normal esophagus.
- Guggulsterone treatment increased apoptosis in Barrett's esophagus-derived cells.
- VDR expression did not significantly differ between groups.
Takeaway
This study found that a receptor called FXR is more active in Barrett's esophagus, and a substance called guggulsterone can help kill bad cells in this condition.
Methodology
The study measured FXR and VDR expression in esophageal biopsies and cell lines using Q-PCR and immunohistochemistry, and assessed apoptosis in cell cultures treated with guggulsterone.
Limitations
Cell cultures were continuously exposed to guggulsterone at a neutral pH, which may not reflect physiological conditions.
Participant Demographics
{"Normal esophagus":{"n":6,"age":"55 ± 7","male_percentage":17},"Reflux esophagitis":{"n":6,"age":"50 ± 9","male_percentage":50},"Barrett's esophagus":{"n":6,"age":"78 ± 3","male_percentage":67},"Esophagus adenocarcinoma":{"n":6,"age":"79 ± 8","male_percentage":67}}
Statistical Information
P-Value
7·10-5
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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