Ponicidin Inhibits Monocytic Leukemia Cell Growth by Induction of Apoptosis
Author Information
Author(s): Liu Jia-Jun, Zhang Yong, Guang Wei-Bin, Yang Hong-Zhi, Lin Dong-Jun, Xiao Ruo-Zhi
Primary Institution: The Third Affiliated Hospital of Sun Yat-sen University
Hypothesis
Does ponicidin induce apoptosis in monocytic leukemia cells?
Conclusion
Ponicidin significantly inhibits the growth of leukemia cells by inducing apoptosis.
Supporting Evidence
- Ponicidin inhibited the growth of U937 and THP-1 cells significantly in a dose- and time-dependent manner.
- Apoptotic cells increased significantly after treatment with ponicidin.
- Survivin and Bcl-2 expressions were down-regulated after ponicidin treatment.
Takeaway
Ponicidin is a substance that can help stop leukemia cells from growing by making them die in a controlled way.
Methodology
The study used MTT assays, flow cytometry, RT-PCR, and Western blot analysis to assess cell viability and apoptosis in leukemia cell lines treated with ponicidin.
Limitations
The study was conducted in vitro, and the effects in vivo remain to be investigated.
Participant Demographics
The study involved monocytic leukemia cell lines U937 and THP-1, and normal peripheral blood monocytes from healthy volunteers.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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