Caspase Cleavage of PM/Scl-75 During Apoptosis
Author Information
Author(s): Schilders Geurt, Raijmakers Reinout, Malmegrim Kelen CR, Walle Lieselotte Vande, Saelens Xavier, Vree Egberts Wilma, van Venrooij Walther J, Vandenabeele Peter, Pruijn Ger JM
Primary Institution: Radboud University Nijmegen
Hypothesis
Is PM/Scl-75 cleaved during apoptosis and what are the implications for autoimmunity?
Conclusion
PM/Scl-75 is specifically cleaved during apoptosis, generating a fragment that remains associated with the exosome complex.
Supporting Evidence
- PM/Scl-75 cleavage was inhibited by caspase inhibitors.
- Cleavage of PM/Scl-75 was observed in various cell types undergoing apoptosis.
- Different caspases cleave PM/Scl-75 at an unconventional site.
Takeaway
When cells die, a protein called PM/Scl-75 gets cut into pieces, and some of these pieces stay with a group of proteins called the exosome, which helps with RNA processing.
Methodology
Jurkat cells were treated with anti-Fas antibody to induce apoptosis, and PM/Scl-75 cleavage was analyzed using Western blotting and caspase inhibitors.
Limitations
The study does not exclude the possibility that cleavage of PM/Scl-75 may not be directly performed by caspases.
Digital Object Identifier (DOI)
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