MicroRNA Profiling in Subventricular Zone after Stroke: MiR-124a Regulates Proliferation of Neural Progenitor Cells through Notch Signaling Pathway
2011

MiR-124a Regulates Proliferation of Neural Progenitor Cells after Stroke

Sample size: 6 publication 10 minutes Evidence: moderate

Author Information

Author(s): Liu Xian Shuang, Chopp Michael, Zhang Rui Lan, Tao Tang, Wang Xin Li, Kassis Haifa, Hozeska-Solgot Ann, Zhang Li, Chen Charles, Zhang Zheng Gang

Primary Institution: Department of Neurology, Henry Ford Hospital, Detroit, Michigan, United States of America

Hypothesis

The study investigates the effect of miR-124a on the Notch signaling pathway in stroke-induced neurogenesis.

Conclusion

Stroke alters miRNA expression in SVZ neural progenitor cells, and miR-124a mediates stroke-induced neurogenesis by targeting the JAG-Notch signaling pathway.

Supporting Evidence

  • Stroke significantly reduced miR-124a expression in neural progenitor cells.
  • Introduction of miR-124a mimics decreased JAG1 expression and Notch signaling.
  • miR-124a increased neuronal differentiation as indicated by higher levels of DCX.

Takeaway

When a stroke happens, a tiny molecule called miR-124a changes in brain cells, which helps control how these cells grow and turn into new brain cells.

Methodology

The study used a rat model of focal cerebral ischemia and analyzed miRNA expression in neural progenitor cells from the subventricular zone.

Limitations

The study primarily focuses on in vitro findings, and further in vivo validation is needed.

Participant Demographics

Adult male Wistar rats (3–4 months old) were used in the study.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0023461

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