Expression of c-erbB-2 Oncoprotein in Human Fetuses
Author Information
Author(s): P. Quirke, A. Pickles, N.L. Tuzi, O. Mohamdeel, W.J. Gullick
Primary Institution: University of Leeds and Imperial Cancer Research Fund Oncology Unit
Hypothesis
The study investigates the pattern of expression of the c-erbB-2 oncoprotein in human fetuses.
Conclusion
c-erbB-2 is widely expressed in human fetuses and occurs earlier than the epidermal growth factor receptor.
Supporting Evidence
- c-erbB-2 was expressed in all three germ layers of the human fetus.
- Expression of c-erbB-2 varied over time during the development of the fetal skeleton.
- Western blotting confirmed expression in placental tissue but not in normal fetal tissues.
Takeaway
This study looked at how a certain protein, c-erbB-2, is found in human babies before they are born, showing it's important for their development.
Methodology
The study used immunocytochemistry and Western blotting on whole mount preparations of human fetuses.
Limitations
The study was limited to well-preserved fetuses, and those with autolysis were discarded.
Participant Demographics
The study involved 11 human fetuses from spontaneous abortions, with gestational ages ranging from 6 to 12 weeks.
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