Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis
2008
New Method for Isolating Membrane Fractions
publication
Evidence: moderate
Author Information
Author(s): Adam Rosalyn M, Yang Wei, Di Vizio Dolores, Mukhopadhyay Nishit K, Steen Hanno
Primary Institution: Children's Hospital Boston and Harvard Medical School
Hypothesis
Can a modified extraction method reduce nuclear contamination in detergent-resistant membrane fractions?
Conclusion
The modified DRM isolation procedure significantly reduces nuclear contamination and is suitable for proteomic analyses.
Supporting Evidence
- The modified method reduced the percentage of nuclear proteins in DRM fractions from 36% to 15%.
- The study confirmed that the modified extraction method yielded fractions comparable to those obtained by conventional methods.
Takeaway
The researchers found a faster way to separate important parts of cells without getting too many unwanted proteins from the nucleus.
Methodology
The study modified a differential detergent extraction method to isolate DRM fractions while minimizing nuclear contamination.
Digital Object Identifier (DOI)
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