High rates of loss of heterozygosity on chromosome 19p13 in human breast cancer
Author Information
Author(s): Oesterreich S, Allred D C, Mohsin S K, Zhang Q, Wong H, Lee A V, Osborne C K, O'Connell P
Primary Institution: Baylor College of Medicine
Hypothesis
Could the nuclear matrix protein SAFB be involved in breast cancer?
Conclusion
The study found a high rate of loss of heterozygosity at the SAFB locus in breast cancer tissues, suggesting it may be a candidate for a breast tumor-suppressor gene.
Supporting Evidence
- LOH was detected at the marker D19S216 in specimens from 29 (78.4%) of 37 informative patients.
- The peak LOH rate occurred at D19S216 near the SAFB locus.
- LOH frequencies ranged from 21.6% to 47.2% at other markers.
Takeaway
Researchers looked at breast cancer tissues and found that a specific gene area had a lot of missing genetic information, which might mean it's important for stopping cancer.
Methodology
Laser capture microdissection was used to isolate DNA from paired primary breast tumor and normal tissue specimens, and loss of heterozygosity was determined using microsatellite markers.
Digital Object Identifier (DOI)
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