OryzaPG-DB: Rice Proteome Database based on Shotgun Proteogenomics
Author Information
Author(s): Helmy Mohamed, Tomita Masaru, Ishihama Yasushi
Primary Institution: Keio University
Hypothesis
The study aims to create a rice proteome database using shotgun proteogenomics to enhance genome annotation.
Conclusion
The OryzaPG database was constructed and is freely available, providing valuable proteomic data for rice genome annotation.
Supporting Evidence
- The database includes approximately 3200 genes covered by identified peptides.
- 40 genes contained novel genomic features based on the proteogenomic analysis.
- The study identified 14,955 unique peptides from the rice genome.
Takeaway
Researchers created a special database to help understand rice genes better by using protein data.
Methodology
The study used shotgun proteomics with nanoLC-MS/MS to analyze undifferentiated cultured rice cells and created a database from the resulting peptide data.
Limitations
The database currently only includes data from undifferentiated cultured rice cells and may not represent all rice tissues.
Participant Demographics
The study focused on undifferentiated cultured rice cells.
Statistical Information
P-Value
< 0.001
Digital Object Identifier (DOI)
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