P-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies
1990

P-glycoprotein Expression in Breast Cancer

Sample size: 29 publication Evidence: moderate

Author Information

Author(s): G.C. Wishart, J.A. Plumb, J.J. Going, A.M. McNicol, C.S. McArdle, T.Tsuruo, S.B. Kaye

Primary Institution: University of Glasgow

Hypothesis

Is P-glycoprotein expressed in primary breast cancer before therapy?

Conclusion

P-glycoprotein is expressed in both epithelial and stromal cells of untreated primary breast cancers.

Supporting Evidence

  • P-glycoprotein was detected in 21 of 29 epithelial cell samples using antibody C219.
  • P-glycoprotein was detected in 26 of 29 stromal cell samples using antibody C219.
  • Immunostaining showed heterogeneous expression in both epithelial and stromal cells.
  • Previous studies have shown varying results for P-glycoprotein expression in breast cancer.

Takeaway

This study looked at breast cancer samples and found a protein that might help cancer cells resist treatment, even before they get any drugs.

Methodology

Immunohistochemistry was used to detect P-glycoprotein expression in frozen sections of breast cancer samples using two monoclonal antibodies.

Limitations

The study's sample size is small and may not represent all breast cancers.

Participant Demographics

All participants were untreated breast cancer patients.

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