Cyclic AMP stimulates platelet-derived growth factor B chain mRNA expression in murine macrophage cell lines
1995
Cyclic AMP Stimulates PDGF B Chain mRNA Expression in Macrophages
publication
Evidence: moderate
Author Information
Author(s): Elizabeth J. Kovacs, Susan VanStedum
Primary Institution: Loyola University
Hypothesis
PGE2 could act in an autocrine/paracrine feed-forward manner to trigger the production of PDGF B chain by macrophages.
Conclusion
Agents that increase intracellular cAMP specifically stimulate the expression of the PDGF B chain gene in macrophages.
Supporting Evidence
- Macrophages do not express interleukin-1 prior to treatment but do express low levels of TNF and PDGF B chain mRNAs.
- Forskolin induced PDGF B chain mRNA expression to levels significantly higher than LPS alone.
- Treatment with PGE2 stimulated PDGF B chain mRNA expression in macrophages.
Takeaway
This study shows that certain substances can help macrophages produce a protein that is important for healing and tissue repair.
Methodology
Murine macrophage cell line ANA-1 was treated with cAMP inducers and LPS, followed by RNA isolation and analysis of mRNA expression.
Want to read the original?
Access the complete publication on the publisher's website